Enner Valencia scored his third goal of the World Cup before being taken off on a stretcher as Ecuador drew with the Netherlands to leave Group A wide open.
PSV Eindhoven forward Cody Gakpo had given the Netherlands the lead after just five minutes and four seconds - the quickest goal so far in Qatar - when he struck a shot beautifully into the top of the net.
But the Dutch struggled to build on their lead, with Ecuador's Pervis Estupinan having a goal disallowed on the stroke of half-time.
Ecuador fans were bouncing with excitement throughout the second half at the Khalifa International Stadium as they equalised just after the break and continued to create chances against a rattled Netherlands side.
The entire Ecuador squad ran over to the corner flag to celebrate with Valencia after he poked in the rebound from Estupinan's parried shot in the 49th minute - and Gonzalo Plata hit the crossbar with a long-range effort shortly after.
Captain Valencia, who scored both goals in Ecuador's victory over hosts Qatar on the opening night of the World Cup, was a constant threat but his evening was cut short due to injury.
The former West Ham and Everton striker went down in the final minute of normal time and was taken off on a stretcher, but was seen hobbling to his seat in the dugout.
The result means three teams from Group A will be battling it out for a place in the last 16 in the final group match - the Netherlands, Ecuador and Senegal - with all three capable of going through as table leaders.
With the Netherlands heavy favourites going into their final group game against bottom side Qatar, all eyes will be on a showdown between Ecuador and Senegal, which will dictate the table order.
"If you want to become world champion then of course you need to improve a lot as some of the opponents we are yet to face is playing at a much higher level," said Netherlands boss Louis van Gaal.
"We simply need to improve our game in terms of ball possession and we are working on that. Whether we'll make it in this tournament I can't guarantee that. But I think so."
Gakpo's opener was the first goal Ecuador had conceded in 701 minutes across all competitions - and a first since March - but the Netherlands struggled to create much as the game wore on.
Estupinan was left frustrated at half-time, with several of his team-mates remaining on the pitch to chat to the officials after his goal was ruled out when team-mate Jackson Porozo was offside and obstructing the view of the goalkeeper.
Van Gaal brought on Memphis Depay at half-time - a planned move as the Barcelona forward continues his return to fitness following injury - but it made little difference as Ecuador were unlucky not to pick up all three points.
Ecuador leader steps up again but at what cost?
The celebrations at full-time were subdued from Ecuador fans after a deserved point against strong opposition.
Valencia, who had an aura about him all evening and had the crowd on their feet on numerous occasions, has scored all three of Ecuador's goals so far at the tournament and they will need him on Tuesday for their crucial group decider with Senegal.
His injury in the final moments of the game will cause concern for Ecuador boss Gustavo Alfaro but otherwise it was a good evening for his side.
"[Valencia] is a top-level player when he wears the Ecuador jersey. I tell him that he will be successful in the World Cup even if he wasn't so much in the qualification campaign," said Alfaro.
"He is going through such a good spell. He is the captain."
Ecuador will be frustrated not to come away with more having had 14 shots in total - including four on target - and limiting the Netherlands to just the one, which was Gakpo's early opener.
Gakpo was on the scoresheet again having scored in the victory over Senegal, only enhancing his reputation in Qatar, but he failed to have an impact on the game afterwards.
The Netherlands have looked ropey in both of their group matches so far but are still in with a strong chance of progressing as group winners.
BDST: 1150 HRS, NOV 26, 2022